Comparative Evaluation of the ID NOWTM Test (Abott) and RT-PCR for the Detection of the SARS-CoV-2 Genome in Travellers
Ndao Malick,
Diagne Babacar,
Diagne Rokhaya,
Niane Moustapha,
Ka Roughyatou
Issue:
Volume 11, Issue 2, June 2023
Pages:
13-16
Received:
17 August 2023
Accepted:
5 September 2023
Published:
14 September 2023
Abstract: Introduction: The persistence of the COVID-19 pandemic, which has become a global public health problem, means that the implementation of effective and affordable diagnostic strategies is essential, particularly in developing countries, to contain the disease. Rapid, reliable and inexpensive molecular or antigenic tests enable early detection of cases and rapid clinical management. The method based on reverse transcription-polymerase chain reaction (RT-PCR) is the benchmark for diagnosing SARS-CoV-2 infections. However, this method requires highly qualified human resources, complex equipment, consumables and reagents that are usually expensive and imported from developed countries. Given these technical and financial constraints and the limited capacity of molecular platforms in developing countries, point-of-care can be considered a very good alternative. The aim of this study was to evaluate the performance of the ID NOWTM COVID-19 test for the detection of SARS-COV-2 from nasopharyngeal swab samples collected in tubes containing viral transport medium compared with RT-PCR. Method: The evaluation was carried out on 59 travellers from whom a nasopharyngeal swab was taken in 3 ml of viral transport medium (VTM). A swab from the ID-NOW kit was dipped into each sample and then deposited in the sample recipient in order to assess the performance of the ID-NOW test compared with RT-PCR. Results: In our study, we found a sensitivity of 92.6% (23/25) and a specificity of 100%. However, 2 false negatives were found with samples that had CT values of 36. No cross-contamination between samples was observed in this study. Conclusion: Our data showed that the ID NOWTM COVID-19 test would be an excellent tool for screening suspected cases in clinical departments.
Abstract: Introduction: The persistence of the COVID-19 pandemic, which has become a global public health problem, means that the implementation of effective and affordable diagnostic strategies is essential, particularly in developing countries, to contain the disease. Rapid, reliable and inexpensive molecular or antigenic tests enable early detection of ca...
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Review Article
The Beneficial Effects of L. reuteri Probiotics in Development of the Toddlers' Immune Systems and Gut Health in Bangladesh
Sultana Begum,
Archana Dev,
Nurul Alam Khan,
Ranjith Debnath,
Mozammel Haque,
Mohammad Amjad Hossain,
Mohammad Sakhawat Hossain,
Rakibul Hasan
Issue:
Volume 11, Issue 2, June 2023
Pages:
17-28
Received:
14 September 2023
Accepted:
4 October 2023
Published:
30 October 2023
Abstract: A well-researched probiotic bacteria called Lactobacillus reuteri (L. reuteri) has the ability to colonize several animals. L. reuteri can be found in the skin, breast milk, urinary tract, gastrointestinal tract, and other parts of the human body. Different people have varying amounts of L. reuteri. There are a number of advantages to L. reuteri. First, L. reuteri has the ability to create compounds that are antimicrobial, such as organic acids, ethanol, and reuteri. L. reuteri's antimicrobial activity enables it to prevent the colonization of pathogenic bacteria and alter the composition of the host's commensal microbiota. Second, L. reuteri can strengthen the immune system of the host. For instance, some L. reuteri strains can enhance the formation and activity of regulatory T cells while suppressing the production of pro-inflammatory cytokines. Third, because L. reuteri has the possibility to fortify the intestinal barrier, its colonization may lessen the number of bacteria that move from the gut lumen to tissues. It has been proposed that microbial translocation across the gut epithelium causes inflammation to start. Therefore, boosting the colonization of L. reuteri may help to alleviate inflammatory illnesses, including those that affect the gut as well as distant organs. Notably, during the past few decades, L. reuteri abundance in humans has declined, and at the same time, inflammatory illness occurrences in toddlers have increased. For the correct developmental growth of toddlers, obvious supplementation, or prebiotic modification of L. reuteri may be an alluring preventative and/or therapeutic pathway counter to inflammatory illnesses.
Abstract: A well-researched probiotic bacteria called Lactobacillus reuteri (L. reuteri) has the ability to colonize several animals. L. reuteri can be found in the skin, breast milk, urinary tract, gastrointestinal tract, and other parts of the human body. Different people have varying amounts of L. reuteri. There are a number of advantages to L. reuteri. F...
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