IL-18 and Anti- P. gingivalis Antibodies in Patients with Diabetes and Chronic Periodontitis
Romero-Herrera Coralia,
De La Rosa-Ramírez Antonio Manuel,
Rosas-Taraco Adrián Geovanni,
Limón-Flores Alberto Yairh,
Arce-Mendoza Alma Yolanda
Issue:
Volume 4, Issue 4, August 2016
Pages:
20-26
Received:
15 September 2016
Accepted:
12 October 2016
Published:
1 November 2016
Abstract: Periodontitis is an important complication in patients with diabetes mellitus. Chronic periodontitis is caused mostly by Porphyromonas gingivalis in Mexico, and by proinflammatory cytokines like IL-1, IL-6, TNFa, and IL-18. The aim of our study was to quantify IL-18 and to detect antibodies against P. gingivalis in gingival crevicular fluid of type 2 diabetes mellitus (T2DM) patients with chronic periodontal disease (PD). We studied a total of 40 patients divided in four groups: group 1 T2DM patients with PD, group 2 T2DM patients without PD, group 3 non-diabetic patients with PD, and a control group of healthy individuals. IL-18 was quantified using a commercial ELISA kit. Detection of IgG and IgM antibodies in gingival crevicular fluid was carried out through ELISA using P. gingivalis semi-purified proteins. Our results showed T2DM patients with chronic PD had low levels of IL-18, whereas non-diabetic patients with chronic PD had the highest. IgM antibodies were detected in diabetic and non-diabetic with chronic PD. IgG antibodies were detected in patients with T2DM and chronic PD, non-diabetic patients with chronic PD, and healthy individuals. In conclusion, IL-18 was detected in low concentrations in gingival crevicular fluid of patients with T2DM and chronic PD. This could be due to a dysregulation of immune-inflammatory responses secondary to diabetes, or by the presence of IL-18 binding protein in gingival fluid. Low levels of IgG antibodies in T2DM patients with PD could be due to the formation of immune complexes, which would explain the greater periodontal damage these patients present.
Abstract: Periodontitis is an important complication in patients with diabetes mellitus. Chronic periodontitis is caused mostly by Porphyromonas gingivalis in Mexico, and by proinflammatory cytokines like IL-1, IL-6, TNFa, and IL-18. The aim of our study was to quantify IL-18 and to detect antibodies against P. gingivalis in gingival crevicular fluid of type...
Show More
