Peripheral Blood Lymphocyte Subsets Counts in Children on Regular Hemodialysis
Manal Abd El-Salam,
Shaimaa Abdelmalik Pessar
Issue:
Volume 3, Issue 1, February 2015
Pages:
1-6
Received:
17 October 2014
Accepted:
31 October 2014
Published:
10 November 2014
Abstract: Background: Hemodialysis (HD) procedure per se as well as disturbances in both innate and adaptive immunity makes HD patients susceptible to infections. Infections are the major cause of morbidity and the second cause of death following cardiovascular events in HD patients. Aim: Was to study lymphocyte subset counts in children with chronic kidney disease on regular hemodialysis in comparison with normal subjects to clarify the abnormalities in cellular immune profile. Patients and methods: The study included 40children with chronic kidney disease on regular hemodialysis and 20 healthy control (HC) children age and sex matched as a control group, they were selected from the pediatric hemodialysis unit and out patients clinic of AL-zahraa hospital ,Al-Azher university during the period from September 2013to June 2014. We examined the number of the peripheral lymphocytes. Also quantification of (T CD3+ & B CD19+) lymphocytes and T cell subsets including T helper CD3+CD4+, T cytotoxic CD3+ CD8+, CD4/CD8 ratio, and NK cells CD3-CD56+ using flow cytometric analysis and correlates their number with clinical and laboratory characteristics. Results: The total peripheral blood lymphocyte count was lower in HD children (2.3 x10³/uL) than HC children (3 x10³/uL), also T lymphocytes CD3+ counts were reduced in HD children (1609.25 ±545.77 / uL) than HC (2114.20± 868.39 /uL), (p=0.008), numbers of CD4+ T cells were not different, but numbers of CD8+ T cells were lower in HD children (600.67±284.92) compared with HC (896.80±573.00) (p < 0.05). Decrease in NK cell counts CD3-CD56+ in HD children (175.35±107.44) in comparison to HC (271.30±169.94). The B lymphocyte count CD19+ was not different in HD children (260±201.6) compared with healthy controls (250.20±122.84). A positive correlation was found between B lymphocytes CD19+ with hemodialysis duration. A negative correlation between NK cell counts and BUN, age and also with patients weight and height were found. Conclusion: The reduced numbers of lymphocyte count, T lymphocytes CD3+counts, CD8+ T cells as well as NK cell in CKD children on regular hemodialysis may favor the frequent occurrence of infections in spit the normal number of the other studied t cell subsets.
Abstract: Background: Hemodialysis (HD) procedure per se as well as disturbances in both innate and adaptive immunity makes HD patients susceptible to infections. Infections are the major cause of morbidity and the second cause of death following cardiovascular events in HD patients. Aim: Was to study lymphocyte subset counts in children with chronic kidney ...
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H. Pylori Prevalence and Its Effect on CD4+ Lymphocyte Count in Active Pulmonary Tuberculosis Patients at Hospitals in Jimma, Southwest Ethiopia
Wakjira Kebede,
Biniam Mathewos,
Gemeda Abebe
Issue:
Volume 3, Issue 1, February 2015
Pages:
7-13
Received:
2 February 2015
Accepted:
27 February 2015
Published:
3 March 2015
Abstract: Background: Helicobacter pylori, a lifelong and typically asymptomatic infection of the stomach, strongly alter gastric immune responses. The present study aimed to survey the prevalence and related risks of H. pylori infection among tuberculosis (TB) patients at hospitals in Jimma City, Southwest Ethiopia. Methods: Comparative cross sectional study was conducted from February to June, 2014. Fifty four PTB patients and an equal number of non TB controls were enrolled. Convenient sampling technique was used to select the study participants. Structured questionnaire was used to collect socio demographic and clinical data. The stool for H. pylori antigen detection and venous blood for CD4+ lymphocyte count was collected. Results: Among 108 study participants, 62 (57.4%) was females. Majority of the study participants, 48 (44.4%) were in the age group of 18-34 years and the mean age of the participants was 37.5 ± 10.7 SD. The prevalence of H. pylori infection among TB patients and non TB controls were 19 (35.2%) and 11 (20.4%), respectively. TB patients with CD4+ lymphocyte count of less than 200Cells/mm3 was more likely to be infected. Conclusion: H. pylori infection among TB patients was significantly higher than non TB controls. Low CD4+ lymphocyte count was found to be associated with high H. pylori infection among TB patients. Further study should be undertaken to reveal the potential pathogenic mechanisms for underlying associations for H. pylori and TB infection.
Abstract: Background: Helicobacter pylori, a lifelong and typically asymptomatic infection of the stomach, strongly alter gastric immune responses. The present study aimed to survey the prevalence and related risks of H. pylori infection among tuberculosis (TB) patients at hospitals in Jimma City, Southwest Ethiopia. Methods: Comparative cross sectional stud...
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