Elaboration of Immunoenzymatic Test-Kit for Total Human IgE Assay and Investigation of Its Analytical Properties
Galkin A. Yu.,
Dugan A. M.
Issue:
Volume 1, Issue 1, April 2013
Pages:
1-6
Abstract: Enzyme-linked immunosorbent assay (ELISA) test-kit has been developed based on complex immuno-chemical, including epitop mapping, characteristics of monoclonal antibodies (MAbs) to human IgЕ. “Sandwich” type ELISA based on usage of different epitop directionality MAbs. It has been founded correlation between affinity of different MAb pairs and its sorbtion-detection ability. Optimal configuration of MAbs in “sandwich” ELISA was follow-ing: 164H10 - 165C12 and 164H10 - 166B7. Cooperative usage of horseradish peroxidase conjugates of MAbs directed to different epitops (165C12-HRP + 166B7-HRP) increased analytical sensitivity of assay and constituted 4.5 IU/ml. Analytical characteristics of developed test-kit were following: dynamic range – from 4.5 to 1600 IU/ml, variation coefficient value during one procedure – 4.2±2.1%, and between procedures – 4.8±2.3%. Presences of human IgG, IgA, IgМ, albumin (1 μg/ml) were not effect on the assay specificity (test concentration of IgE – 50 IU/ml).
Abstract: Enzyme-linked immunosorbent assay (ELISA) test-kit has been developed based on complex immuno-chemical, including epitop mapping, characteristics of monoclonal antibodies (MAbs) to human IgЕ. “Sandwich” type ELISA based on usage of different epitop directionality MAbs. It has been founded correlation between affinity of different MAb pairs and its ...
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Interferon Gamma (IFN-γ) and Soluble Interleukin-2 Receptor (sIL-2R): Combined Diagnostic Markers of Tuberculous Pleural Effusion
Mohamed S. Abdel-Latif,
Lobna A. Abou-Shamaa,
Eglal A. El-Sherbini,
Mohamed S. M. Afifi
Issue:
Volume 1, Issue 1, April 2013
Pages:
7-13
Abstract: Background: pleural TB is a diagnostic challenge because of its nonspecific clinical manifestation. The efficiency of conventional laboratory method and the reliance on pleural biopsy have motivated the evaluation of alternative diagnostic strategies. The objective of the current study is to evaluate the diagnostic efficiency of IFN-γ and sIL-2R levels in pleural effusion for differential diagnosis of tuberculous pleurisy. Methods: estimated levels of IFN-γ and sIL-2R were compared with the result of conventional PCR and Z-N staining used for detection of M. tuberculosis DNA and acid fast bacilli screening of pleural effusion, respectively. Involved study population included 60 patients with pleural effusion, divided into two groups: Tuberculous group (40 patients: 7 confirmed TB and 33 probable TB cases) and control group (20 patients: 10 cases due to malignancy and 10 cases due to heart failure). Results: our results showed that IFN-γ and sIL-2R levels are significantly higher in tuberculous group than in control group. Conclusion: current study suggested that measurement of IFN-γ and sIL-2R in pleural effusion could be less invasive and quicker diagnostic tools of TPE compared to conventional microbiological diagnostic methods.
Abstract: Background: pleural TB is a diagnostic challenge because of its nonspecific clinical manifestation. The efficiency of conventional laboratory method and the reliance on pleural biopsy have motivated the evaluation of alternative diagnostic strategies. The objective of the current study is to evaluate the diagnostic efficiency of IFN-γ and sIL-2R le...
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