Volume 4, Issue 6, December 2016, Page: 68-72
Prevalence of Anti-A and Anti-B Haemolysins in Group O Blood Donors at the National Blood Transfusion Center of Abidjan, Côte d'Ivoire
Goran-Kouacou Amah Patricia Victorine, Immunology and Allergology Department, Unit of Training and Research Medical Sciences in Abidjan, Félix Houphouët-Boigny University, Abidjan, Côte d’Ivoire; Immunology and Hematology Service, University Hospital of Cocody, Abidjan, Côte d’Ivoire
Siransy Kouabla Liliane, Immunology and Allergology Department, Unit of Training and Research Medical Sciences in Abidjan, Félix Houphouët-Boigny University, Abidjan, Côte d’Ivoire; National Blood Transfusion Center, Abidjan, Côte d’Ivoire
Adou Adjoumanvoulé Honoré, Immunology and Allergology Department, Unit of Training and Research Medical Sciences in Abidjan, Félix Houphouët-Boigny University, Abidjan, Côte d’Ivoire; Immunology and Hematology Service, University Hospital of Cocody, Abidjan, Côte d’Ivoire
Yéboah Oppong Richard, Immunology and Allergology Department, Unit of Training and Research Medical Sciences in Abidjan, Félix Houphouët-Boigny University, Abidjan, Côte d’Ivoire; Immunology and Hematology Service, University Hospital of Cocody, Abidjan, Côte d’Ivoire
Guina Denise Affoué Blassonny, Immunology and Hematology Service, University Hospital of Cocody, Abidjan, Côte d’Ivoire
Sékongo Yassongui Mamadou, National Blood Transfusion Center, Abidjan, Côte d’Ivoire; Hematology Clinic, University Hospital of Yopougon, Abidjan, Côte d’Ivoire
N’Guessan Koffi, Immunology and Allergology Department, Unit of Training and Research Medical Sciences in Abidjan, Félix Houphouët-Boigny University, Abidjan, Côte d’Ivoire; Immunology and Hematology Service, University Hospital of Cocody, Abidjan, Côte d’Ivoire
Dassé Séry Romuald, Immunology and Allergology Department, Unit of Training and Research Medical Sciences in Abidjan, Félix Houphouët-Boigny University, Abidjan, Côte d’Ivoire; Immunology and Hematology Service, University Hospital of Cocody, Abidjan, Côte d’Ivoire
Received: Dec. 11, 2016;       Accepted: Dec. 26, 2016;       Published: Jan. 18, 2017
DOI: 10.11648/j.iji.20160406.14      View  4235      Downloads  171
Abstract
ABO blood-group system is characterized by the constant presence in the plasma of natural anti-A and anti-B antibodies (regular, agglutinating, class IgM) corresponding to the antigens absent on the membrane of the red blood cell. In addition to these natural antibodies, there may be immune antibodies (called haemolysins) in response to different types of immunological stimuli. These anti-A and anti-B haemolysins are capable of triggering the complete cascade of complement leading to haemolytic accidents in the case of non-isogroup ABO transfusion. Our objective was to determine the frequency of IgG anti-A, anti-B and anti-A + B haemolysins in group O blood donors at the national blood transfusion center of Abidjan. Our retrospective cross-sectional study was conducted at the national blood transfusion center of Abidjan and that investigated anti-A, anti-B and anti-A + B haemolysins in the sera of 191 group O blood donnors, aged 18-65 years, using the heat technique with direct agglutination of the serum to be tested by red blood cells A1 and B papained. The prevalence of IgG haemolysins was 35.08% (10.47% anti-A, 15.71% anti-B and 8.9% anti-A + B). The average age of our population was 32,9 years [18-63 years] with a male / female sex ratio of 4.97. Haemolysin levels were higher in men (27.75%) than in women (7.33%) (not a significant difference, p= 0.44). Age distribution showed a high haemolysin level in the 25-29 age group (significant difference, p = 0.001), with variable rate ranging from 2.1% to 6.3%. Ideally, group O blood should only be transfused to group O subjects, except in emergency situations where iso-group blood is not available. Since the technique used in our study to investigated haemolysins was a qualitative method, we could not have the title of the various haemolysins in order to assess the real risk of an immunological accident after such a blood transfusion.
Keywords
Haemolysins, Blood Donors, Group O, Côte d'Ivoire
To cite this article
Goran-Kouacou Amah Patricia Victorine, Siransy Kouabla Liliane, Adou Adjoumanvoulé Honoré, Yéboah Oppong Richard, Guina Denise Affoué Blassonny, Sékongo Yassongui Mamadou, N’Guessan Koffi, Dassé Séry Romuald, Prevalence of Anti-A and Anti-B Haemolysins in Group O Blood Donors at the National Blood Transfusion Center of Abidjan, Côte d'Ivoire, International Journal of Immunology. Vol. 4, No. 6, 2016, pp. 68-72. doi: 10.11648/j.iji.20160406.14
Copyright
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reference
[1]
Lefrère JJ, Rouger Ph. Transfusion sanguine, 4ème édition, Masson, 2011.
[2]
Louati N, Cherif J, Ben Amor I, Rekik H, Gargouri J. Recherche des hémolysines chez les donneurs de sang. Journal de l’Information Médicale de Sfax Juin-Déc 2008; 15-16: 17-9.
[3]
Uko EK, Erhabor O, Ahmed HM, Isaac IZ, Abdulrahaman Y, Wase A, Ezimah A, Aghedo F Ikuenbor DB, Udomah FP, Iweke IP, Adias TC. Prevalence of high titre alpha and beta haemolysins among blood donors in Sokoto, North Western Nigeria. International Journal of Medical Sciences and Health Care Dec 2013; 1 (11): 1-7.
[4]
Giraud Ch. Korach JM, Andreu G, Lacaze C, Vaicle M, Schooneman F, Guillevin L. Les bases immunologiques de la transfusion. Transfusion Clinique et Biologique Juillet 2002; 9 (3): 163-7.
[5]
Kulkarni AG, Ibazebe R, Fleming AF. High frequency of anti-A and anti-B haemolysins in certain ethnic groups of Nigeria. Vox Sanguinis 1985; 48 (1): 39-41.
[6]
Olawumi HO, Olatunji PO. Prevalence and titre of alpha and beta haemolysins in blood group ‘O’ donors in Ilorin,” African Journal of Medicine and Medical Sciences Dec 2001; 30 (4): 319-21.
[7]
Kagu MB, Sagir G. Ahmed, Aisha A. Mohammed, Waheed K. Moshood, Mohammed B. Malah, Jimoh M. Kehinde. Anti-A and Anti-B haemolysins amongst group “O” voluntary blood donors in Northeastern Nigeria. Journal of Transfusion 2010; Vol 2011, Article ID 302406, 3p.
[8]
Oyedeji OA, Adeyemo TA, Ogbenna AA, Akanmu AS. Prevalence of anti A and anti B hemolysis among blood group O donors in Lagos. Nigerian Journal of Clinical Practice, May-Jun 2015; 18 (3): 328-32.
[9]
Rouger Ph, Salmon Ch. Les allohémagglutinines du système ABO. Revue Française de Transfusion et d’Immuno-hématologie 1977; 20 (4): 623-5.
[10]
Fopa D, Tagny CT, Tebeu PM, Ndoumba A, Mbanya D. Recherche et titrage des hémolysines anti-A et anti-B chez les femmes en période du postpartum immédiat au Centre Hospitalier et Universitaire de Yaoundé. Africa Sanguine 2013; 16 (2): 4-8.
[11]
Ugah U, Ibekailo S, Mbamagu D. Rate of Haemolysins among Blood Donors in Abakaliki, Ebonyi State, Nigeria. Global Journal of Medicine Researches and Studies 2014; 1 (3): 61-5.
Browse journals by subject