Volume 4, Issue 4, August 2016, Page: 20-26
IL-18 and Anti- P. gingivalis Antibodies in Patients with Diabetes and Chronic Periodontitis
Romero-Herrera Coralia, Faculty of Odontology, Autonomous University of Nuevo León, Monterrey, Nuevo León, México
De La Rosa-Ramírez Antonio Manuel, Periodontics, University of Monterrey (UDEM), Monterrey, Nuevo León, México
Rosas-Taraco Adrián Geovanni, Immunology Department, Faculty of Medicine, Autonomous University of Nuevo León, Monterrey, Nuevo León, México
Limón-Flores Alberto Yairh, Immunology Department, Faculty of Medicine, Autonomous University of Nuevo León, Monterrey, Nuevo León, México
Arce-Mendoza Alma Yolanda, Immunology Department, Faculty of Medicine, Autonomous University of Nuevo León, Monterrey, Nuevo León, México
Received: Sep. 15, 2016;       Accepted: Oct. 12, 2016;       Published: Nov. 1, 2016
DOI: 10.11648/j.iji.20160404.11      View  3353      Downloads  115
Periodontitis is an important complication in patients with diabetes mellitus. Chronic periodontitis is caused mostly by Porphyromonas gingivalis in Mexico, and by proinflammatory cytokines like IL-1, IL-6, TNFa, and IL-18. The aim of our study was to quantify IL-18 and to detect antibodies against P. gingivalis in gingival crevicular fluid of type 2 diabetes mellitus (T2DM) patients with chronic periodontal disease (PD). We studied a total of 40 patients divided in four groups: group 1 T2DM patients with PD, group 2 T2DM patients without PD, group 3 non-diabetic patients with PD, and a control group of healthy individuals. IL-18 was quantified using a commercial ELISA kit. Detection of IgG and IgM antibodies in gingival crevicular fluid was carried out through ELISA using P. gingivalis semi-purified proteins. Our results showed T2DM patients with chronic PD had low levels of IL-18, whereas non-diabetic patients with chronic PD had the highest. IgM antibodies were detected in diabetic and non-diabetic with chronic PD. IgG antibodies were detected in patients with T2DM and chronic PD, non-diabetic patients with chronic PD, and healthy individuals. In conclusion, IL-18 was detected in low concentrations in gingival crevicular fluid of patients with T2DM and chronic PD. This could be due to a dysregulation of immune-inflammatory responses secondary to diabetes, or by the presence of IL-18 binding protein in gingival fluid. Low levels of IgG antibodies in T2DM patients with PD could be due to the formation of immune complexes, which would explain the greater periodontal damage these patients present.
Antibodies, Porphyromonas gingivalis, Cytokines, Type 2 Diabetes, Periodontitis
To cite this article
Romero-Herrera Coralia, De La Rosa-Ramírez Antonio Manuel, Rosas-Taraco Adrián Geovanni, Limón-Flores Alberto Yairh, Arce-Mendoza Alma Yolanda, IL-18 and Anti- P. gingivalis Antibodies in Patients with Diabetes and Chronic Periodontitis, International Journal of Immunology. Vol. 4, No. 4, 2016, pp. 20-26. doi: 10.11648/j.iji.20160404.11
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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